- 10X Restriction Enzyme Reaction Buffer 3
- 基因表达载体和病毒
- Prigrow CI Medium
- 1kb Plus Opti-DNA Marker
- 10X Restriction Enzyme Reaction Buffer 2
- 抗体
- PriCoat Flasks
- 细胞生物学
- Prigrow VI Medium
- 2019冠状病毒疾病(Coronavirus Disease 2019);
- 10X Universal Restriction Enzyme Reaction Buffer
- Prigrow X Series Medium for T5403
- 1kb Opti DNA标记
- miRNA一体化cDNA合成试剂盒
- 100bp Opti-DNA Marker
- 普锐斯系列T0768培养基
- 高保真™ 保真度DNA聚合酶
- PriCoat T75烧瓶,过滤器盖
- 10X Restriction Enzyme Reaction Buffer 1
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- 分子生物学和PCR
Description | This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes. |
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SKU | K095 |
System | Non-Viral Vector |
Product Applications |
|
Vector Map | pNV-EF1a-Cas9-2A-GFP |
Unit quantity | 10 μg |
Promoter | EF1a |
Cas Type | Nuclease |
Cas Origin | spCas9 |
Cas9 Expression | Constitutive Cas9 |
Note | abm"s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant). |
Shipping Conditions | Shipped at ambient temperature. |
Bacterial Selection | Ampicillin |
Format | Plasmid |
Appearance | Liquid |
Quality Control | Restriction Enzyme Digest and Sequencing |
Shelf Life | 1 year (when at -20°C or below in a non-frost free freezer) |
Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |