Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1).

Molecular Structure: A soluble fusion protein consisting of the mature extracellular (173aa) domain of human CD40: epptacrekqylinsqccslcqpgqklvsdctefteteclpcgesefldtwnrethchqhkycdpnlglrvqqkgtsetdtictceegwhctseacescvlhrscspgfgvkqiatgvsdticepcpvgffsnvssafekchpwtscetkdlvvqqagtnktdvvcgpqdrlr

+linker: gt

fused to murine IgG2a Fc (233aa): eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpg

Predicted monomeric molecular weight of 45.6 kd. The dimeric protein runs at ~95 kD in SDS-PAGE.

Transfectant Cell Line: CHO

Functional Application: CD40-muIg fusion protein blocks binding of anti-human CD40 to Raji human tumor cells.

References:

1. T.A. Foy, et al, (1996) Annu Rev Immunol 14: 591-617.

2. M.E. Ozaki, et al, (1997) J Immunol 159: 214-221

3. N. Hubbard, T. R. Torgerson, et al. (2016) Blood :blood-2015-11-683235; doi: https://doi.org/10.1182/blood-2015-11-683235

Ancell防鼠CD45R（B220）纯化（含抗生素）同种型：  大鼠IgM免疫原：   RAW112细胞信息：抗体RA3-3A1与B220（CD45R）的胞外表位结合，B220是T200的220 kD变异体，存在于B细胞和B细胞前体上。CD45R参与了涉及运动性和枝晶形成的信号传导途径（2）。