Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphatidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages.

Isotype: Murine IgG1

Immunogen: Human polymorphonuclear leukocytes

Specificity: Antibody 3G8 recognizes both allelic forms of human CD16 (1, 4). It is cross reactive with cynomolgus (7) as well as Rhesus monkey(10) CD16.

Functional Application: Antibody 3G8 blocks binding of complexed IgG to CD16 (2, 5, 9), and has been used to block ADCC in Vitro (11).It is not suitable for Western blot. Ancell 3G8 F(ab‘2) has been used to upregulate Mac-1 expression in neutrophils (8).

References:

1. H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279.

2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597.

3. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234.

4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814.

5. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.

6. S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-4259.

7. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.

8. Z Xiao, S Neelamegham, et al. (2008) Blood 112: 1091-1100.

9. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

10. Choi EI, Reimann KA, et al. (2008) Immunology 124(2): 215-22 PMID: 18201184

11. H Tanaka, M Yasukawa, et al. (2016) DOI: 10.1158/1078-0432 CCR-15-2714. PMID: 27091408

Ancell防鼠CD45R（B220）纯化（含抗生素）同种型：  大鼠IgM免疫原：   RAW112细胞信息：抗体RA3-3A1与B220（CD45R）的胞外表位结合，B220是T200的220 kD变异体，存在于B细胞和B细胞前体上。CD45R参与了涉及运动性和枝晶形成的信号传导途径（2）。