Human IgG1k from human myeloma plasma is used as a negative control(1,2) for work involving human IgG1 isotype antibodies or recombinant proteins.

The molecular weight of this myeloma derived protein has varied for some lots; running at about 135 kD in SDS-PAGE, compared to the expected 150 kD for normal human IgG1. This was due to a smaller heavy chain, which ran at approximately 40 kD when reduced.

References

1) J Shen, PS Low, et al. (2014) J Leuk Biol 96: 563-570. PMID: 25015955

2) C E Green, S I Simon, et al.(2003) Am J Physiol - Cell Physiol 284(3): C705-C717.

3) S Watanabe, S G Vasudevan, et al.(2015) J Virol 89(11): 5847-61.

Ancell防鼠CD45R（B220）纯化（含抗生素）同种型：  大鼠IgM免疫原：   RAW112细胞信息：抗体RA3-3A1与B220（CD45R）的胞外表位结合，B220是T200的220 kD变异体，存在于B细胞和B细胞前体上。CD45R参与了涉及运动性和枝晶形成的信号传导途径（2）。