QUANTITY: 100 ug each of 8 Biotinylated reagents : Sufficient for use with approximately 108 Ficoll prepared PBMC
Information: Low Density Granulocytes(LDG) are a unique sub population of cells associated with Systemic Lupus Erythematosus (SLE) etiology. Isolated low density granulocytes (LDG) have capacity to interfere with differentiation of Endothelial precursors. While LDG have impaired phagocytic function, they are cytotoxic to vascular endothelium. LDG also exhibit enhanced extracellular trap (NET) ability. This cell population may play a significant role in SLE progression.
This cocktail of 8 Biotinylated monoclonal antibodies has been used in conjunction with anti-Biotin MACS beads to quickly isolate and characterize this unusual population of neutrophils from ficoll prepared, RBC-lysed Systemic Lupus Erythematosus patient blood. Purity of isolated cells was determined as >95% CD10+, CD15+/CD14lo.
References: 1) M.F. Denny, S Yalavarthi, M.J. Kaplan, et al. (2010) J Immunol 184(6): 3284-3297 2) Carmona-Rivera C, MJ Kaplan (2013) Semin Immunopathol 35(4):455-63. doi: 10.1007/s00281-013-0375-7.
Components: (see individual product inserts for each biotinylated mAb)
anti-CD3/Biotin
anti-CD7/Biotin
anti-CD19/Biotin
anti-CD79b/Biotin
anti-CD56/Biotin
anti-MHC Class II/Biotin
anti-CD86(B7-2)/Biotin
anti-CD235a(Glycophorin A)/Biotin
Ancell防鼠CD45R（B220）纯化（含抗生素）同种型： 大鼠IgM免疫原： RAW112细胞信息：抗体RA3-3A1与B220（CD45R）的胞外表位结合，B220是T200的220 kD变异体，存在于B细胞和B细胞前体上。CD45R参与了涉及运动性和枝晶形成的信号传导途径（2）。
