INFORMATION: The FOXP3 molecule is a 50–55 kD transcription factor also known as IPEX, JM2, Forkhead box 3, and Scurfin. Defects in this gene can result in lethal autoimmune disease (2). When used with cell surface markers CD4 and CD25(IL-2R), FOXP3 is a useful marker for identifying T regulatory cells. Functionally, FOXP3 is thought to mediate oxidative phosphorylation, enabling Treg to function in a lower oxygen environment (3).

In EIA, clone ANCFX2D7 binds to full length recombinant FOXP3 and to a FOXP3(R1-R3) construct (aa 1–198) but not to a FOXP3(R1) construct (aa 1-70), suggesting that its epitope is within regions 2 and 3 (aa 71–198).

ANCFX2D7 binds to nuclear FOXP3 found constitutively in a 0.5 – 3% sub population of fixed, permeabolized CD4+ peripheral blood lymphoid cells in FACS.

FOXP3 References:

1) G Roncador, A H Banham, et al. (2005) Eur J Immunol 35(6): 1681-1691. PMID: 15902688.

2) S Hori, S Sakaguchi, (2004) Microbes and Infection 6(8): 745-751.

3) U H Beier, W W Hancock, et al. (2016) J Immunol 196(1 suppl 211.3).

Ancell防鼠CD45R（B220）纯化（含抗生素）同种型：  大鼠IgM免疫原：   RAW112细胞信息：抗体RA3-3A1与B220（CD45R）的胞外表位结合，B220是T200的220 kD变异体，存在于B细胞和B细胞前体上。CD45R参与了涉及运动性和枝晶形成的信号传导途径（2）。