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商品描述
| Product Name | 5(6) - FAM[5 - (and - 6) - Carboxyfluorescein] *UltraPure Grade* *Mixed Isomers* |
| Size | 10 g |
| Catalog # | AS-81002-10 |
| US$ | $249 |
| Purity | >90% |
Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
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| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | 1. Biswas S, et al.J Colloid Interface Sci 271, 157-62;(2004).2. Ma LY, et al.Spectrochim Acta A Mol Biomol Spectrosc 60, 1865-72;(2004).3. Kermis HR, et al.Analyst 128, 1181-6;(2003).4. Kermis HR, et al.Biotechnol Prog 18, 1047-53; (2002).5. Ozkan P, et al. R Biochim Biophys Acta 1572, 143-8; (2002).6. Marechal X, et al. Photochem. Photobiol 70, (5) 813-819; (1999). |
| Molecular Weight | 376.32 |
| Molecular Formula | C21H12O7 |
| Spectral Properties | Abs/Em = 494/519 nm |
| Solvent System | DMF or DMSO |
| Product Citations | Carroll, NJ. et al. (2014). Measurement of flow velocity and inference of liquid viscosity in a microfluidic channel by fluorescence photobleaching. Langmuir 30, 4868. |
