This kit is optimized to detect rat alpha-Synuclein (α-Synuclein) in cell and tissue lysate, and in body fluids such as plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.

• OneStep Convenient Format
• Pre-coated and pre-blocked 96-well strip plate
• Ready-to-use substrate solution and other assay components
• One step assay (samples and detection antibody are addedsimultaneously)
• 1 hour assay time at room temperature (excluding incubationtime)
• MinimalSample Size
• Requires only 10-20 μl of cell and tissue lysate or body fluids
• HighSensitivity
• Detects 5 pg/ml of α-Synuclein (calculated as three standarddeviations from the blank)
• BroadDynamic Range
•  8-500 pg α-Synuclein/ml in the assayed sample