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商品描述
| Product Name | MMP Colorimetric Substrate IAc - PLG - SCH[CH2CH(CH3)2] - CO - LG - OC2H5 |
| Size | 5 mg |
| Catalog # | AS-27097 |
| US$ | $494 |
| Purity | % Peak Area By HPLC ≥ 95% |
An exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4-dithiodipyridine or Ellmans Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate. | |
| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C |
| References | Ref: Weingarten, H. and J. Feder, Anal. Biochem. 147, 437 (1985); Weingarten, H. et al. Biochem. 24, 6730 (1985). |
| Molecular Weight | 655.9 |
| Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5 | |
| Sequence(Three-Letter Code) | Ac - Pro - Leu - Gly - SCH[CH2CH(CH3)2] - CO - Leu - Gly - OC2H5 |
