| Product Name | SensoLyte ® Homogeneous AFC Caspase - 3/7 Assay Kit500 assays (96 - well plate) |
| Size | 1 kit |
| Catalog # | AS-71114 |
| US$ | $315 |
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors. | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) Poster |
| Product Citations | Freise, C. & U. Querfeld(2014). Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34. Pharmacol Res doi: http://dx.doi.org/10.1016/j.phrs.2014.04.013.Johnson, JG. et al. (2014). Caspase-like activity during aging and cell death in the toxic dinoflagellate< i> Karenia brevis. Harmful Algae 31, 41.Meek, RL. et al. (2013). Glomerular cell death and inflammation with high-protein diet and diabetes. Nephrol Dial Transplant doi: 10.1093/ndt/gfs579.Freise, C. et al. (2013). The inhibitor of Ca2+-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB. Invest New Drugs. doi:10.1007/s10637-012-9879-6Freise, C. et al. (2011). A hepatoprotective Lindera obtusiloba extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines. BMC Complementary Altern Med 11, 39.Cheung, M. et al. (2010). An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo. Mol Cancer 10.1186/1476-4598-9-28.Freise, C. et al. (2009). An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes. J Nutr Biochem 10.1016/j.jnutbio.2009.09.013.Rueh, M. et al. (2009). Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern. J Nutr Biochem 20, 597.Ou, D. et al. (2005). Synergistic inhibition of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in human pancreatic β cells by Bcl-2 and X-linked inhibitor of apoptosis. Hum Immunol 66, 274. |
