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商品描述
| Product Name | Caspase 3 (Apopain) Substrate 1m, fluorogenicAc - DEVD - AMC |
| Size | 5 mg |
| Catalog # | AS-25262-5 |
| US$ | $159 |
AMC (7-amino-4-methylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm. Caspase-3 substrate with Km = 9.7 µM; Caspase-7 substrate with Km = 11 µM | |
| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87; Garcia-Calvo M, et al. (1998). Inhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem 273, 32608-13; Thornberry NA, et al. (1997). A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J Biol Chem 272, 17907-11; Rano TA, et al. (1997). A combinatorial approach for determining protease specificities: application to interleukin-1 beta converting enzyme (ICE). Chem Biol 4, 149-55; Margolin N, et al. (1997). Substrate and inhibitor specificity of interleukin-1 ?-converting enzyme and related caspases. J Biol Chem 272, 7223-8; Mizushima N, et al. (1996). Ceramide induces apoptosis via CPP32 activation. FEBS Lett 395, 267-71; Faubion WA, et al. (1999). Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas. J Clin Invest 103, 137-45. |
| Molecular Weight | 675.5 |
| Ac-DEVD-AMC | |
| Sequence(Three-Letter Code) | Ac - Asp - Glu - Val - Asp - AMC |
| Product Citations | Stoka, V. et al. (2006). Differential regulation of the intrinsic pathway of apoptosis in brain and liver during ageing. FEBS Lett 580, 3739.Gukovsky, I. et al. (2003). Curcumin ameliorates ethanol and nonethanol experimental pancreatitis. AJP-GI 284, G85.Lin-Shiau, SY. et al. (2003). Induction of apoptosis by rosemary polyphenols in HL-60 cells. Orient Foods Herbs chapter doi: 10.1021/bk-2003-0859.ch008.Gukovskaya, AS. et al (2002). Cholecystokinin induces caspase activation and mitochondrial dysfunction in pancreatic acinar cells: Roles in cell injury processes of pancreatitis. J Biol Chem 277, 22595.Yusef, K. et al. (2002). Trophoblast differentiation modulates the activity of caspases in primary cultures of term human trophoblasts. Ped Res 52, 411. |
