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Active Bio/innuSCRIPT One Step RT-PCR Probe Kit – 100 reactions/innuSCRIPT One Step RT-PCR Probe Kit – 100 reactions/ANL845-RT-7000100

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货号:ANL845-RT-7000100
浏览量:120
品牌:Active Bio
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商品描述

Product Details– Contains thermostable reverse transcriptase (50° C to 55° C) and an effective RNase inhibitor– Reverse transcription followed by real-time PCR in a single step– Highly sensitive– Universally applicable: standard and fast cyclers, GC- and AT-rich templates– Simple handling and superior reproducibility– Fast amplification rate for Ct values that can be detected early

The innuSCRIPT One Step RT-PCR Probe Kit is a ready-to-use, 2x master mix uniting reverse transcription with subsequent qPCR in a single reaction vessel. Positive test results are already available for the reaction mix on a variety of commercially available real-time thermal cyclers. The innuSCRIPT One Step RT-PCR Probe Kit is based on cDNA synthesis and subsequent real-time PCR using specific probe-detection technologies. The kit composition was validated in such a way that each RNA template can be quantified, including mRNA, total RNA and viral sequences. Detection is also highly efficient for targets for which the number of copies is extremely small. Adding RNA template and primer to the reaction are the only preparation steps involved. The final step is to add PCR-grade water to achieve the final volume.

SpecificationsConcentration2x master mix

PS-Mg2+ solutionThe concentration of MgCl2 in the master mix has been optimized, eliminating the need for adding more MgCl2.

Active Bio的AccuPower®黄金多重PCR PreMix,12 x 8条试管(20ul)特点和优点多重PCR的20个复用扩增产物在单管中产生仅具有一个反应特异性通过应用PyroHotStart技术(包括焦磷酸酶)将非特异性扩增减至最少,从而通过消除每个循环中产生的PCR抑制剂焦磷酸盐来最大化效率。方便使用PyroHotstart技术容易地扩增多个模板从1至20,与能够引发PCR过程中的任何的PCR条件缩短计算时间只是通过将模板DNA及其corrosponding引物组稳定性增强的稳定性从而允许长期储存通过在PCR反应混合物中的稳定剂和干燥再现通过在ISO 9001质量体系下的一个批次系统中进行批量生产进行批量生产获得可重复的实验结果,并对每个批次进行全面的质量控制,然后以统一质量的产品形式提供