Rabbit polyclonal to beta Tubulin - Loading Control Suitable for: WB, ICC/IF, IHC-P, IP Reacts with: Human Isotype: IgG 长期批量供应 —— 采用重组技术，可实现快速生产 首次实验即可成功 —— 经过大量验证确认了特异性 符合伦理标准 —— 产品不含动物成分 This antibody detects a single clean band at 50kD representing beta Tubulin. This band is significantly reduced by using peptide blocking. Human预测可用于: Mouse, Rat, Chicken, Pig, Xenopus laevis, Zebrafish, Chinese hamster Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.(Peptide available as ab20775) 常规说明 The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As 存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. 存储溶液 pH: 7.40Preservative: 0.02% Sodium azideConstituent: PBSBatches of this product that have a concentration The Abpromise guarantee Abpromise™承诺保证使用ab6046于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 IHC-P (1) Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). IHC-PUse a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. 组织特异性 Ubiquitously expressed with highest levels in spleen, thymus and immature brain. 疾病相关 Cortical dysplasia, complex, with other brain malformations 6Skin creases, congenital symmetric circumferential, 1 翻译后修饰 Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. All lanes : Anti-beta Tubulin antibody - Loading Control (ab6046) at 1/500 dilutionLane 1 : HeLa Cell lysateLane 2 : A431 Cell lysateLane 3 : MCF7 Cell lysateLane 4 : 293 Cell lysateLane 5 : HeLa Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/mlLane 6 : A431 Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/mlLane 7 : MCF7 Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/mlLane 8 : 293 Cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 50 kDaExposure time: 10 seconds ICC/IF image of ab6046 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1 g/ml) overnight at +4 C. The secondary antibody (green) was ab150081 Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 M.The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100%methanol (5 min). ICC/IF image of ab6046 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 5 g/ml) overnight at +4 C. The secondary antibody (green)was DyLight 488 goat anti-rabbit IgG - H L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 M. Beta Tubulin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Tubulin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6046.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 50kDa: beta Tubulin. IHC image of beta Tubulin staining in human liver carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6046, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 发表研究结果有使用 ab6046？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab6046 被引用在 817 文献中. Velasco K et al. Functional evaluation of 16 SCHAD missense variants: Only amino acid substitutions causing congenital hyperinsulinism of infancy lead to loss-of-function phenotypes in vitro. J Inherit Metab Dis 44:240-252 (2021).PubMed: 32876354 Zhang W et al. The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation. Gene 769:145228 (2021).PubMed: 33096182 Li Z et al. Potent aneugenicity of 1-methylpyrene in human cells dependent on metabolic activation by endogenous enzymes. Arch Toxicol 95:703-713 (2021).PubMed: 33057863 Clapper E et al. The regulation of bcr-abl in hypoxia is through the mTOR pathway. Leuk Lymphoma 62:967-978 (2021).PubMed: 33251904 Pavlikova N et al. Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach. PLoS One 15:e0229430 (2020).PubMed: 33104727 View all Publications for this product Blocking step Serum as blocking agent for 30 minute(s) Concentration: 2.5% Temperature: 25 C Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 22 C Blocking step Serum as blocking agent for 20 minute(s) Concentration: 10% Temperature: 20 C Blocking step Serum as blocking agent for 30 minute(s) Concentration: 10% Temperature: 20 C Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) Concentration: 3% Temperature: 4 C Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: rt C Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 3% Temperature: 25 C Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 4% Temperature: 23 C Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 20 CPlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com

>>> 更多资讯详情请访问蚂蚁淘商城