Isotype: IgG Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB 存储溶液 Preservative: 0.01% Gentamicin sulphateConstituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate 纯化说明 This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. 常规说明 The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support. The Abpromise guarantee Abpromise™承诺保证使用ab6789于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。 IHC image ofbeta actinstaining inhuman colonformalin fixed paraffin embedded tissue section*.The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 g/ml overnight at +4 C. An HRP-conjugated secondary (ab6789,1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.The inset negative control image is secondary-only at 1/500 dilution.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilutionAll lanes : Cytosolic fraction prepared from murine brain tissueLysates/proteins at 10 µg per lane.SecondaryAll lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Observed band size: 194 kDa why is the actual band size different from the predicted?Exposure time: 1 minute ab49969 ImmunoprecipitatingHA Tagin HEK-293T whole cell lysate. 25 g of cell lysate was incubated with primary antibody (1/2500 inlysis buffer) and matrix (Protein A/G) for 6 hours at 4 C. For western blottinga HRP-conjugated goat anti-mouse IgG H L (ab6789) (1/1000) was used to confirm successful immunoprecipation.See Abreview ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504. To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on. Click here to view the general protocols 发表研究结果有使用 ab6789？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab6789 被引用在 746 文献中. Fan J et al. Oncolytic herpes simplex virus and temozolomide synergistically inhibit breast cancer cell tumorigenesis in vitro and in vivo. Oncol Lett 21:99 (2021).PubMed: 33376532 Lu N et al. miRNA-490-3p promotes the metastatic progression of invasive ductal carcinoma. Oncol Rep 45:706-716 (2021).PubMed: 33416185 Sinitski D et al. Cross-kingdom mimicry of the receptor signaling and leukocyte recruitment activity of a human cytokine by its plant orthologs. J Biol Chem 295:850-867 (2020).PubMed: 31811089 Dong Y et al. Generation of a Monoclonal Antibody against D-Dimer Using HTS-Based LiCA. SLAS Discov 25:310-319 (2020).PubMed: 31560248 Hu W et al. Blueberry anthocyanin-enriched extract ameliorates transverse aortic constriction-induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice. Mol Med Rep 21:454-462 (2020).PubMed: 31746378 View all Publications for this product Secondary antibody (ab 6789) was used at a dilution of 1:5000 in 5% BSA. Placental lysate was used. primary antibody was purchased for beta actin from pierce antibody. gel: 10%, 200 V for 35 min.transfer 17 V for 35 min. using Biorad TURBO Blocking 5% BSA. Thank you for kindly confirming these details. I am sorry these vials of antibody have not worked for this customer.As requested, we are pleased to arrange a free of charge replacement. I can confirm that 1 vial each of ab6789, ab72439 and ab77450 have been added to your order number [BO] NL-396-Dt:-11-12-2012. This has Abcam order reference 1224924.I would like to reassure you that this free of charge replacement vial is also covered by our Abpromise guarantee. Should the customer still be experiencing difficulties with the new vials, or if you have any further questions, please do not hesitate to let me know.Thank you for your help and cooperation with this case. Please do not hesitate to contact me if you need anything further. Read More Thank you for your message and for providing this further information.I am sorry to hear the suggestions made have not improved the results on this occasion.I appreciate the time the customer has spent on these experiments, and would like to offer a refund or credit note in compensation (providing the items have been purchased in the 6 month guarantee period). I am sorry I notice I do no think we have received the order numbers in this case? To enable us to proceed with this, I would appreciate if you could please confirm the order numbers and date of purchase?Please note that it is important for us to receive the order number as soon as possible when a complaint is submitted, preferably within the first email. This is vital for our investigation of the case and for our quality monitoring purposes.Thank you for your continued cooperation. I look forward to hearing from you with the order information and details of how you would like to proceed. Read More Thank you for your response. I have spoken to my colleague and we can certainly ship the replacement vial directly to your company Qafqazbiotex Co to Georgia. Once you place a new order, we can add this item (free of charge)(ab6789) to your document and despatch them together. However, we are unable to send the product via France. Let me know if you have any further question and when the new order will be ready. Read More Thank you for your response and for confirming the order number. I could arrange a new vial of ab6789 as a free of charge replacement or a credit note. Please could you discuss it with your troubled customer and let me know how you wish to proceed with this enquiry. I look forward to hearing from you soon. Read More Thank you for confirming the batch number. In my previous e-mail, I have also asked you to provide your Abcam Order number (or your Purchase Order Number) with the date of purchase. We do not have any order in our system from Georgia so it would be interesting to know how you purchased this product. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. I look forward to hearing from you soon. Read More Thank you for your enquiry regarding ab6789. In order to take this complaint further, could you please confirm the batch number and your Abcam Order number (or your Purchase Order Number) with the date of purchase. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. I look forward to hearing from you soon. Read More Thank you very much for your call earlier this month and for your patience. We now have FITC-conjugated streptavidin available as ab136222- https://www.abcam.com/Streptavidin-protein-FITC-ab136222.html I apologize that this took longer than expected, but I hope that this information will be useful. Please let me know if you have any questions and I\'ll be happy to help. Read More Thank you very much for your call yesterday. As we discussed, we are currently adding several streptavidin-fluorophore conjugates to the catalog and I will let you know as soon as the streptavidin-FITC is ready for sale. I expect this to happen within the next couple of days. Please let me know if you have any questions or if there is anything else that we can do for you. Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com

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