Cat.No.147318 | 200 µl hybridoma supernatant, lyophilized. For reconstitution add 200µlH2O, then aliquot and store at -20°C until use. |
Applications | WB:not recommended (see remarks) IP:not recommended (see remarks) ICC:1 : 200galleryIHC:1 : 200(see remarks) galleryIHC-P/FFPE:not tested yet |
Clone | GpmAb7a |
Subtype | IgG2 (κ light chain) |
Immunogen | Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555) |
Epitop | Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555) |
Reactivity | Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.Other species not tested yet. |
Specificity | Specific for the brain specific 93kDa splice variant. (K.O.verified) |
Remarks | WB:Clone 3B11 (cat. no. 147 111) highly recommended. IP:Clone 3B11 (cat. no. 147 111) highly recommended. IHC:For best results use the protocol of Schneider Gasser etal., 2006.This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells. |
Datasheet | 147_318.pdf |