Cat.No.135311 | 100 µg purified IgG, lyophilized.Albumin and azide were added for stabilization. For reconstitution add 100µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications | WB:1 : 500 up to 1 : 2000 (AP staining)(see remarks) galleryIP:yes(see remarks) ICC:1 : 100galleryIHC:1 : 100galleryIHC-P/FFPE:1 : 100 up to 1 : 500galleryELISA:yes (see remarks) |
Clone | 317D5 |
Subtype | IgG2a (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634) |
Epitop | Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634) |
Reactivity | Reacts with: rat (Q62634), mouse (Q3TXX4).Other species not tested yet. |
Specificity | Specific for VGLUT 1. (K.O.verified) |
matching control protein/peptide | 135-3P |
Remarks | WB:This antibody yields stronger signals in Western blot experiments than cat.no. 135511 but is less sensitive than cat.no. 135011 and the polyclonal VGluT1 antibodies. IP:Coupling to protein A is recommended for IP, since covalent coupling to activated sepharose leads to considerable loss of activity. ELISA:Suitable as capture antibody for sandwich-ELISA with cat.no. 135303 as detector antibody (protocol for sandwich-ELISA).VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
Datasheet | 135_311.pdf |