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Synaptic Systems(SYSY)/VGLUT 1 - 135 311/135 311

价格
¥700000.00
货号:135311
浏览量:87
品牌:SYSY
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商品描述
Cat.No.135311100 µg purified IgG, lyophilized.Albumin and azide were added for stabilization. For reconstitution add 100µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
ApplicationsWB:1 : 500 up to 1 : 2000 (AP staining)(see remarks) galleryIP:yes(see remarks) ICC:1 : 100galleryIHC:1 : 100galleryIHC-P/FFPE:1 : 100 up to 1 : 500galleryELISA:yes (see remarks)
Clone317D5
SubtypeIgG2a (κ light chain)
ImmunogenRecombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)
EpitopEpitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
ReactivityReacts with: rat (Q62634), mouse (Q3TXX4).Other species not tested yet.
SpecificitySpecific for VGLUT 1. (K.O.verified)
matching control protein/peptide135-3P
Remarks

WB:This antibody yields stronger signals in Western blot experiments than cat.no. 135511 but is less sensitive than cat.no. 135011 and the polyclonal VGluT1 antibodies.

IP:Coupling to protein A is recommended for IP, since covalent coupling to activated sepharose leads to considerable loss of activity.

ELISA:Suitable as capture antibody for sandwich-ELISA with cat.no. 135303 as detector antibody (protocol for sandwich-ELISA).VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Datasheet135_311.pdf
SYSY我们希望您的反馈!我们很高兴地宣布,Synaptic Systems已与pAbmAbs开始合作,   以促进抗体领域的透明性和科学再现性!pAbmAbs是一个独立的非商业性抗体审查平台,可帮助研究人员浏览抗体的世界,找到最适合其方法的抗体。 许多研究实验室在性能不如 广告宣传的抗体上花费了大量资金。浪费时间,金钱和宝贵的资源只是在这种情况下可能发生的许多后果中的少数。更为严重的后果是生成了错误的,误导性的数据,这些数据指导研究人员和科学界朝错误的方向前进;导致将更多的时间和金钱花费在基于人工制品的研究上。生物科学目前面临的重现性危机也部分归因于特异性差或变异性差的抗体。我们认为,只有研究人员和抗体供应商采取协作开放的态度,才能改善这种情况并提高研究抗体的整体质量。