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Cat.No.146211 | 100µgpurifiedIgG,lyophilized.Azidewasaddedbeforelyophilization.Forreconstitutionadd100µlH2Otogeta1mg/mlsolutioninPBS.Thenaliquotandstoreat-20°Cuntiluse. |
Applications | WB:notrecommended(seeremarks)IP:nottestedyetICC:yes(methanolfixation)IHC:1:500upto1:1000(seeremarks)galleryIHC-P/FFPE:nottestedyet |
Clone | 299E7 |
Subtype | IgG1(κlightchain) |
Immunogen | RecombinantproteincorrespondingtoAA336to455fromratGlycinereceptorβ(UniProtId:P20781) |
Epitop | aa336-355 |
Reactivity | Reactswith:rat(P20781),mouse(P48168),monkey.Otherspeciesnottestedyet. |
Specificity | Specificfortheglycinereceptorβ-subunit. |
Remarks | WB:DetectionofoverexpressedproteinispossIBLe.Endogenouslevelsarehardlydetectable. IHC:Mildfixationfor10-15minwithcold2%PFAsolutionisrecommended.Include0,5%tritonX100inallblockingandantibodyincubationsteps. |
Datasheet | 146_211.pdf |