Cat.No.160103 | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen.Albumin was added for stabilization. For reconstitution add 50µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications | WB:1 : 1000 (AP staining)galleryIP:not tested yetICC:1 : 200 up to 1 : 500(see remarks) galleryIHC:1 : 500galleryIHC-P/FFPE:1 : 500galleryEM:yes |
Immunogen | Recombinant protein corresponding to AA 1 to 196 and 1 to 176 and 1 to 177 from human Homer1/2/3 (UniProt Id: .) |
Reactivity | Reacts with: human (Q86YM7, Q9NSB8, Q9NSC5), rat (Q9Z214, O88801, Q9Z2X5), mouse (Q9Z2Y3, Q9QWW1, Q99JP6), zebrafish.Other species not tested yet. |
Specificity | AA 1-120 were used for affinity purification which are homologous in homer 1, 2, and 3. |
Remarks | ICC:PFA fixation recommended. Methanol fixation leads to a complete loss of the signal. |
Datasheet | 160_103.pdf |