Epigenomicinformationprovidesgreaterinsightintogeneregulation,butiscomplicatedbythefactthatindividualcells(eventhoseofthesametype)mayhavedistinctepigenomes.OurICELL8platformoffersahigh-throughputsolutionthatenablesthegenerationofsingle-cellATAC-seq(scATAC-seq)librariesfrom>1,000cellsina4–5-houron-chipautomatedworkflow.

ATAC-seq:generategenome-widechromatinaccessibilitymaps

ATAC-seqstandsforAssayforTransposase-AccessIBLeChromatinusingsequencing.ATAC-seqisusedforthegenome-widemappingofchromatinaccessibilityandemploysahyperactiveTn5transposasetoselectivelyinsertDNAadaptersintoaccessiblechromatinloci.Adapter-ligatedDNAfragmentsarethenamplifiedbyPCRandusedtogeneratesequencing-readylibraries.ThismethodrepresentsapowerfultoolforinvestigatingthedynamicsofgeneexpressionregulationandallowsforthesimultaneousexaminationoftheaccessibilityofgenomicDNAtotranscriptionfactorsandnucleosomeposition(s)inregulatorysites,whileprovidingagenome-widemapofchromatinaccessibility.

ATAC-seqontheICELL8platform

OurICELL8platformenablesasimple,relativelyinexpensivescATAC-seqworkflowthatoffersa20-foldincreaseinthroughputcomparedtopriormethods.OurtechnologyallowsthegenerationofATAC-seqdatafrom>1,000viablecellsisolatedandselectedautomaticallywiththeICELL8Single-CellSystem(Cat.No.640000)andCellSelectSoftware.ThechipisthentransferredtotheICELL8ChipCyclerwheretagmentation,indexing,andPCRamplificationisperformedon-chip.Followingamplification,full-lengthampliconsarepooledoff-chip,purified,andthepurifiedCDNAisamplifiedtoyieldthefinalsequencing-readylibraries.