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商品描述
| Cat.No.147108 | 50 µg purified IgG, lyophilized. For reconstitution add 50µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Applications | WB:yes (see remarks) IP:yes (see remarks) ICC:1 : 500galleryIHC:1 : 500(see remarks) galleryIHC-P/FFPE:not tested yet |
| Clone | HumAb7a |
| Subtype | IgG (κ light chain) |
| Immunogen | Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555) |
| Epitop | Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555) |
| Reactivity | Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.Other species not tested yet. |
| Specificity | Specific for the brain specific 93kDa splice variant. (K.O.verified) |
| Remarks | This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with human specific sequences. The antibody can, therefore, be used with standard anti-human secondary reagents. WB:Clone 3B11 (cat. no. 147 111) highly recommended. IP:Clone 3B11 (cat. no. 147 111) highly recommended. IHC:For best results use the protocol of Schneider Gasser etal., 2006.Alternatively, AGR (10 mM citrate, pH 6.0, overnight at 60°C) can be applied. |
| Datasheet | 147_108.pdf |
