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商品描述
| Cat.No.147018 | 200µlhybridomasupernatant,lyophilized.Forreconstitutionadd200µlH2O,thenaliquotandstoreat-20°Cuntiluse. |
| Applications | WB:notrecommended(seeremarks)IP:notrecommended(seeremarks)ICC:1:1000galleryIHC:1:500upto1:1000(seeremarks)galleryIHC-P/FFPE:nottestedyet |
| Clone | RBMAb7a |
| Subtype | IgG1(κlightchain) |
| Immunogen | NativeProteincorrespondingtoAA1to768fromratGephyrin(UniProtId:Q03555) |
| Epitop | N-terminusofgephyrin,surroundingphospho-serines268and270. |
| Reactivity | Reactswith:human(Q9NQX3),rat(Q03555),mouse(Q8BUV3),pig,goldfish,zebrafish.Otherspeciesnottestedyet. |
| Specificity | Specificforthebrainspecific93kDasplicevariant.(K.O.verified) |
| Remarks | WB:Clone3B11(cat.no.147111)highlyrecommended. IP:Clone3B11(cat.no.147111)highlyrecommended. IHC:ForbestresultsusetheprotocolofSchneiderGasseretal.,2006.AlternativelyAGR(10mMcitrate,pH6.0,overnightat60°C)canbeapplied.ThisantibodyisachimericantibodybasedonthewellknownmonoclonalmouseantibodymAb7a.Theconstantregionsoftheheavyandlightchainshavebeenreplacedwithrabbitspecificsequences.Theantibodycanthereforebeusedwithstandardanti-rabbitsecondaryreagents.Theantibodyhasbeenexpressedinmammaliancells. |
| Datasheet | 147_018.pdf |
