Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells.
Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells.Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., BME, EDTA, DTT, glycerol, TCEP, etc.; see compatibility table), and when performing tandem purification of his-tagged proteins.
The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein-binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. Miniprep, maxiprep, 24-well-plate, 96-well-plate, and large-volume formats are available toaccommodate a range of input volumes. Furthermore, the short residence time of protein samples greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.
