The DNA Blunting Kit allows the conversion of 3" and 5" overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3"→5" exonuclease and 5"→3" polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can then be ligated into a vector using the same optimized buffer system employed in our: DNA Ligation Kit, Mighty Mix; TaKaRa DNA Ligation Kit LONG; and Quick DNA ligation kit products. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without the need for further DNA purification.