The Refolding CA Kit takes advantage of a novel artificial chaperone technology in an easy-to-use two-step process to optimize refolding conditions of inclusion body proteins. Optimization of protein refolding conditions results in correct protein folding and restoration of protein activity. One can also use this kit to solubilize inclusion body proteins.
The Refolding CA (Cycloamylose) Kittakes advantage ofa novel artificial chaperone technologyin an easy-to-use two-step process to optimize refolding conditions of inclusion body proteins. Optimization of proteinrefoldingconditions results in correct protein foldingand restoration of protein activity. One can also use this kit to solubilize inclusion body proteins.
The smaller-size Refolding CA Kit is supplied with guanidine hydrochloride (GdmCl) and dithiothreitol (DTT) for protein unfolding, four different surfactants that can be added independently to the unfolded protein solution (protecting the proteinfrom molecular aggregation), and highly polymerized cycloamylose (CA), an artificial chaperone, for surfactant removal and recovery of protein activity. Overnight incubation of the CA-treated protein is followed by a quick 10-min centrifugation step and collection of the refolded protein-containing supernatant.
The Refolding CA Large Kit isintended for large-scale protein refolding after reaction conditions have been optimized using the smaller Refolding CA Kit. The large kit consists of only denaturant and CA.
The artificial chaperone technology in the Refolding CA Kit is licensed from NFRI, BTRAI and Ezaki Glico Co, Ltd.
