Mung Bean Nuclease acts as a preferential DNA and RNA endonuclease,hydrolyzing single-stranded DNA and RNA into 5"-phosphate- and 3"-hydroxyl-containing products. If used at very high concentrations,Mung Bean Nuclease isalso capable of degrading double-stranded DNA, RNA or DNA/RNAfragments from both ends. Due to its single-stranded DNA and RNA endonuclease activity, Mung Bean Nucleaseis often usedduring transcript mapping, duplex or hybridDNA and RNA blunt ending, and cDNA preparation.