The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA from very low amounts of input RNA that is compromised, has a low RIN value, and/or lacks a poly(A) tail. The kit was specifically designed for RNA-Seq applications involving FFPE or laser-captured samples, and works with as little as 200 pg and up to 10 ng of rRNA-depleted material. It outperforms existing methods in terms of gene-body coverage, data reproducibility, read mappability, and ERCC correlation.For upstream rRNA removal from10-100 ng of input total RNA, we recommend theRiboGone - Mammalian kit.

cDNA produced with this kit is compatible with low-input NGS library preparation kits, including those for Illumina and Ion Torrent.