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Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.
Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.
Probe qPCR Mix, with UNG is supplied as a 2X premix containing Tli RNase H and Uracil N-Glycosylase (UNG). A heat-resistant Tli RNase H can minimize PCR inhibition by degrading residual mRNA in reactions using cDNA templates, while UNG can avoid false positives due to carryover contamination by degrading previously amplified PCR products with this kit.