Reverse transcription of mRNA followed by PCR (RT-PCR) is a common cDNA cloning method; however, it can be difficult to obtain full-length cDNA using this method. Rapid amplification of cDNA ends (RACE) helps overcome this problem by using a gene-specific primer for reverse transcription followed by amplification with specifically designed oligonucleotide primers. The 5'-Full RACE Core Set takes advantage of inverse PCR to amplify and identify unknown 5' cDNA ends, and also containsall the reagents required for reverse transcription, DNA-RNA hybrid degradation, circularization and subsequent amplification of the single-stranded cDNA. Because the 5'-Full RACE Core Set uses AMV Reverse Transcriptase XL to maximize first-strand cDNA length,it is recommended for use with Takara Taq, Takara Ex Taq, or Takara LA Taq DNA Polymerase enzymes.