The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain a multiple cloning siteat the lacZ" region that enables recombinantplamids to be verified via blue/white colony screeningusing agar plates containing IPTG and X-Gal.Expression oftarget DNA is enabled by the presence of a lac promoter in the cloning vectors. ThepUC18 and pUC19 plasmids are suitable for dideoxy DNA sequencing using M13 primers. To sequence pUC18 and pUC19 plasmids containing large DNA insertions, utilize the Deletion Kit for Kilo-Sequencing (Cat. # 6030).