Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp.,and purified from E. coli. This endonuclease enzyme cancomplete digestionofall types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures andeven in samples placedon ice. As a result, Cryonase Cold-Active Nuclease is frequently usedduring DNA digestion of mixed samples, such as thosethat include heat-labile proteins.