Generally, low molecular weight peptides and synthetic peptides do notspontaneously attach to 96-well microtiter ELISA plates. The Peptide Coating Kit is designed to allow optimal peptide plate coatingonto96-well plates. The Peptide Coating Kit also includes reaction buffer, 96-well microplates, and blocking solution designed specifically for subsequent ELISA.

Principle

The free carboxyl groups of peptides used for plate coating can be covalently linkedvia reaction with the water-soluble peptide coupling reagent l-ethyl-3-(3-(dimethylamino)-propy1)carbodiimide (EDC). These peptides can subsequently belinkedto amino groups thatare attachedto the surface of microtiter plate wells. The end result is a population ofpeptides directly coating the surface of the microtiter platevia peptide bonds.