Listeria is a gram-positive bacillus with flagellated spores. While eight species of bacteria are included in the genus Listeria, only Listeria monocytogenes is thought to act as a major human disease pathogen. Symptoms of listeriosis include a headache and fever, and the disease can cause meningitis, septicemia, endocarditis, pneumonia, and multiple other illnesses. Listeria monocytogenes has drawn attention as a major food-poisoning pathogen. The PCR Listeria monocytogenes Detection Kit (multi-PCR) China conforms to the industry standard of Entry-Exit Inspection and Quarantine of the People"s Republic of China (method "SN/T 0184.2-2006 food monocytogenes Listeria detection methods - Part 2: multiplex PCR method developed for the detection of Listeria monocytogenes by multiplex PCR"). Use this kit for multiplex PCR to detect two Listeria monocytogenes target genes.

The kit includes Tks Gflex DNA Polymerase, an enzyme derived from an archaebacterium in the genus Thermococcus. Tks Gflex DNA Polymerase can effectively inhibit non-specific binding of the enzyme with template DNA, thereby improving fidelity and providing excellent amplification. The enzyme system also includes a proprietary elongation factor to enhance priming specificity (patent pending). Therefore, high specificity and very high-speed amplification can be achieved with a wide range of template concentrations. Tks Gflex DNA Polymerase facilitates amplification not only of routine templates but also challenging targets such as GC-rich or AT-rich templates, as well as crude samples that contain PCR-inhibiting agents. It is formulated to support hot-start PCR. The TaKaRa PCR Listeria monocytogenes Detection Kit (multi-PCR) China allows simple and rapid detection of Listeria monocytogenes with high sensitivity and specificity.