Traditional methods of plant cell wall disruption that rely on tissue pulverization in liquid nitrogen using a mortar and pestle are not needed whentaking advantage of the Plant DNA Isolation Reagent. Thisplant DNA extraction kitmakes preparing multiple samples at the same time more convenient. Furthermore, improvements in reagent composition reduce heat treatment timeto only 15 minutes. In its entirety, theplant DNA extraction process can be completed within 30 minutes.Purified plant genomic DNA can be used directly for downstream applications such as PCR or restriction endonuclease digestion.
Traditional methods of plant cell wall disruption that rely on tissue pulverization in liquid nitrogen using a mortar and pestle are not needed whentaking advantage of the Plant DNA Isolation Reagent. Thisplant DNA extraction kitmakes preparing multiple samples at the same time more convenient. Furthermore, improvements in reagent composition reduce heat treatment timeto only 15 minutes. In its entirety, theplant DNA extraction process can be completed within 30 minutes.Purified plant genomic DNA can be used directly for downstream applications such as PCR or restriction endonuclease digestion.
Our Plant DNA Isolation Reagent is a ready-to-use plant DNA extractionkit that includes benzyl chloride forextraction ofplant genomic DNA. Benzyl chloride causes disintegration of plant cell walls by benzylation of the cellulose hydroxyl groups and other components of the plant cell wall. This kit takes advantage of benzyl chloride and uses it in a procedure that involves freezing the plant tissue, then thawing and grindingit with a pipette tip in a microcentrifuge tube. The freeze-thawing and grinding effectively degrade the plantcell wall.
