Cloning Enhancer removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning. PCR samples treated with Cloning Enhancer yield significantly more recombinant clones and since treatment with Cloning Enhancer occurs in the same tube as the PCR reaction, it is less likely to result in UV damage or nicking. Avoiding a separate cleanup process also minimizes the risk of losing PCR product during purification.