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商品描述
| Cat.No.152103 | 50µgspecificantibody,lyophilized.Affinitypurifiedwiththeimmunogen.AlbuminwasaddedforstABIlization.Forreconstitutionadd50µlH2Otogeta1mg/mlsolutioninPBS.Thenaliquotandstoreat-20°Cuntiluse. |
| Applications | WB:1:1000(seeremarks)galleryIP:nottestedyetICC:1:500upto1:5000galleryIHC:yes(seeremarks)IHC-P/FFPE:nottestedyet |
| Immunogen | RecombinantproteincorrespondingtoAA856to888frommouseCa2+channelP/Q-typeα-1A(Cav2.1)(UniProtId:P97445) |
| Reactivity | Reactswith:rat(P54282),mouse(P97445).Otherspeciesnottestedyet. |
| Specificity | SpecificforCa2+channelα-1A.(K.O.verified) |
| Remarks | WB:Duetoitslargesize,thisantibodyrequiresspecialgel-electrophoresisandWesternblotprotocolsforvisualizationbyimmunoblotting.Excellentresultscanbeobtainedwiththe4-12%TRIS-glycinegrADIentgelsofanamedorNuPageTRIS-acetategelsfromInvitrogen.Thisproteintendstoaggregateafterboiling,makingitnecessarytorunSDS-PAGEwithnon-boiledsamples. IHC:Antibody2,cat.no.152203,ishighlyrecommendedforthisapplication. |
| Datasheet | 152_103.pdf |
