The chloramphenicol-resistant pUC vectors pHSG396 and pHSG398contain the chloramphenicol resistance gene, the pMB1-derived origin of replication (ori), andthe beta-galactosidase coding gene lacZ. These vectors also contain a pUC18-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verifiedthrough culture plates containing IPTG and X-Gal. High target-geneexpression isenabled by the presence of thelac promoteron both the pHSG396 and pHSG398vectors.

Both pHSG 396 and pHSG 398canbe analyzed via dideoxy DNA sequencing using an M13 primer, and large recombinant chloramphenicol resistant pUC vectors may be analyzed using the Deletion Kit for Kilo-Sequencing.