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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/NitroPure Nitrocellulose Transfer Membrane, 0.22 um , LC7033/20cm x20cm, sheet ,10/PK/LC7033-200
商品描述
| Pore Size | Both WestPure PVDF and NitroPure nitrocellulose membranes are commonly available in two different pore sizes, 0.22 and 0.45 um. Membranes with a pore size of 0.22 um are generally recommended for poteins with a molecular weight of less than 15 kDa, while WestPure PVDF 0.45 um membranes are suitable for most Western blotting applications(5 kDa - 400 kDa protein). |
|---|---|
| Membrane Integrity | PVDF membranes are less fragile than nitrocellulose and generally a better choice for experiments that require stripping and re-probing of the membrane. |
| Detection Method | WestPure PVDF and NitroPure Nitrocellulose are both well suited for chemiluminescence and fluorescence detection methods, however WestPure PVDF must be used for fluorescence detection to avoid high background resulting from auto fluorescence of standard PVDF membranes. It is recommended that you cut a small sample of membrane and image it both wet and dry, to check for autofluorescence and background. |
| Transfer Buffer | Transfer buffer must contain methanol when using nitrocellulose membranes. Although WestPure PVDF membranes must be pre-wetted with methanol, they can be used with methanol-free transfer buffer. |
NitroPure Nitrocellulose Transfer Membrane is the membrane of choice for all protein or immunoblotting applications.The high sensitivity of NitroPure Nitrocellulose Transfer Membrane ensures excellent results in all transfers, especially in protein blotting.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
