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Gendepot/Puredown Protein A-Agarose, BSA-Binding Site Blocked, P9201/P9201-015-1.5ml

价格
¥1080.00
货号:P9201-015-1.5ml
浏览量:98
品牌:Gendepot
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商品描述
Reduced BackgroundMinus albumin-binding region to reduce non-specific binding.
ConvenientReady to use for immunoprecipitation procedures
Low CostCost less than other competitor\\\'s Protein A/G-Agarose.
Concentration4% cross-linked agarose
Binding Capacity> 35mg/ml Human IgG
ApplicationImmunoprecipitation (IP)
Protein A is a cell wall component and four high affinity binding sites capable of binding specificaly to the Fc region of immunoglobin molecules from several species. The Protein A molecule is heat-stable and retains its native conformation, when exposed to denaturing reagents such as 4M urea, 4M thiocynate and 6Mguanidine hydrochloride.
Puredown Protein A is covalently coupled to highly cross-linked agarose bead.This product is provided preblocked with fatty acid free bovine serum albumin and have been extensively washed to reduce-non-specific binding. Formulations are ready to use and 10ul of primary antibody can be used in standard immuno-precipitaion procedures.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。