Peak Wavelengths | 650 nm, unstopped, blue reaction product450 nm, stopped, yellow reaction product |
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Reaction Temperature | Room temperature |
Substrate Concentration | 2.08 mmol/L |
This high sensitivity liquid substrate for peroxidase consists of Tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase in ELISA systems which is stable for long-term storage providing sensitivity greater than OPD.
This reagent is available as a single component, ready-to-use reagent. When used in conjunction with our proprietary TMB Stop Buffer (TSB), the resulting color product will not fade or darken for several hours.