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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/ProteinSeeker Mammalian Cell Lysis Solution , P9400/P9400-010-100 ml
商品描述
| High yield | Total protein extraction from cultured cells and tissue samples. |
|---|---|
| Fast and Simple | No sonication or freeze-thaw cycles |
| Convenient | room temperature process |
| Compatible with Downstream Applications | - 1D and 2D electrophoresis- Western blotting and immunoprecipitation- Affinity Purification- EMSA, reporter gene assays and enzyme activity |
ProteinSeekerMammalian Cell Lysis Solution is a ready-to-use solution designed for extraction of native, non-denatured proteins from mammalian cultured cells or tissue samples.The procedure, which is based on lysis with mild detergents at room temperature, is fast and simple: no need for sonication, freeze thaw cycles or scraping of adherent cells. The procedure yields proteins in their native, non-denatured functional state. Therefore, isolated proteins can be used directly in many proteomic and enzymatic activity applications, including immunoprecipitation, affinity purification, reporter gene assays and electrophoretic mobility shift assays (EMSA). Isolated proteins are also compatible with quantification assays such as Bradford and Lowry.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
