D-Glucose | 3151 mg/L |
---|---|
L-Glutamine | 365 mg/L |
HEPES | 3574.5 mg/L |
Sodium Pyruvate | 55 mg/L |
Phenol Red | 8.1 mg/L |
Since Harry Eagle"s first reports, studies have been underway to determine the media requirements for individual cell types. The medium found to be most satisfactory for these studies as a 1:1 mixture of Dulbecco"s Modified Eagle"s Medium (DMEM) and Ham"s F-12 Nutrient Mixture. To help compensate for the buffering capacity lost with the removal of serum, HEPES has been added in some modifications at a final concentration of 15 mM.