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Gendepot/1kb PCR Ranger DNA Marker, 75bp-To-20kb, D1109/250ul/D1109-025

价格
¥480.00
货号:D1109-025
浏览量:98
品牌:Gendepot
服务
全国联保
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商品描述
Contents1kb PCR Ranger DNA Marker is composed of fifteen chromatography-purified individual DNA fragments in 10mM Tris-HCl (pH7.6 at 25C ), 1mM EDTA, 5% Glycerol.1.66% Sucrose, 0.01% Xylene cyanol FF, 0.01% Cresol Red, 0.013% Bromophenolblue, 0.05% Orange G.
The 1kb PCR Ranger DNA Marker is a premixed, ready-to-use molecular weight marker containing 4 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
This product is designed for sizing and approximate quantification of wide range double-stranded DNA fragments on agarose and polyacrylamide gels.
The marker is composed of fifteen chromatography-purified individual DNA fragments (in base pairs) : 20000, 10000, 7000, 5000, 4000, 3000, 2000, 1500, 1000, 700, 500, 400, 300, 200, 75. The 5000, 1500 and 500 base pair bands have increased intensity to serve as reference points.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。