Trypsinisapancreaticserineproteasewithsubstratespecificitybaseduponpositivelychargedlysineandargininesidechains.Itisderivedfroma34kDainactiveprecursorzymogen,trypsinogen,afterenzymaticremovalofann-termial6-aminoacidleadersequenceresultinginthe23.8kDatrypsinmolecule.The
optimumpHis8.0.Trypsinisinhibitedbyorganophosphoruscompoundssuchasdiisopropylfluorophosphateandnaturalinhibitorsfrompancreas.Soybean,limabean,andeggwhitearealsosourceofnaturalinhibitors.TrypsincleavesamideandesterbondsofArgandLys.TheGenDEPOT’sSequencingGradeTrypsinistreatedwithL-(tosylamido-2-phenyl)ethylchloromethylketonetoinhibitcontaminatingchymotrypticactivity,chemicallymodifiedtopromotestABIlityandfurtherpurifiedtoremoveautolysisfragments,resultinginahighlystabletrypsinproductresistanttoautolysiswhileretainingspecificity. 
