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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/Medium 199 With Earles Salts with Phenol Red, CM038/500 ml/CM038-050
商品描述
| D-Glucose | 1000 mg/L |
|---|---|
| Glutamine | 100 mg/L |
| HEPES | No HEPES |
| Menadione Sodium Bisulfite | No Menadione Sodium Bisulfite |
| Phenol Red | 20mg/L |
In an effort to produce a totally defined nutritinal source for cells, Morgan, Morton, and Parker, in 1950, developed what has become known as Medium 199. It was found that the growth of explanted tissue could be measured in Medium 199. However, long-term cultivation of cells required serum supplement. Currently, it is widely used in virology and vaccine production. It is also used in the culture of many non-transformed cells.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
