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商品描述
| GLuc-M2 sequence | KPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDGSLSTPPTPSPSTPPTGLNDIFEAQKIEWHE |
|---|---|
| Gluc-M2 | For optimal results and a long lasting glow kinetic please use following Gaussia-M2 sequence(Welsh et al. , Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence,Biochemical and Biophysical Research Communications 389 (2009) 563–568) |
LucyQ Gaussia Luciferase Assay Kitcontains reagents for measuringGaussialuciferase activity in mammalian cell culture media and lysates. When used with GaussiaVectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of anyGaussialuciferases or derivatives that use coelenterazine as a substrate.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
