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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
| Recommended | For blue/white colony screening, we recommend using X-Gal / IPTG Solution at a 500-1000X concentration. |
|---|---|
| Application | Blue/white colony screening |
X-Gal / IPTG Solution is a ready-to-use solution of 40mg/ml X-Gal (5-bromo-4-chloro-3-indolyl ß-D-galactopyranoside) and 24mg/ml IPTG (Isopropyl b-D-thiogalactopyranoside) in a non-hazardous and non-toxic solvent (N-methylpyrrolidon). The X-Gal / IPTG Solution saves time and money, since no extra sterilization is necessary, and it can be used directly from the freezer. The solution provides optimal blue/white colony screening and is compatible with all commonly used lacZ expression systems in bacteria, yeast and mammals.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
