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Gendepot/Goat anti-Guinea Pig IgG(H+L), 0.5mg/ml , SA004/SA004-500-500ul

价格
¥2200.00
货号:SA004-500-500ul
浏览量:79
品牌:Gendepot
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商品描述
Specificity/Cross ReactivityTested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with guinea pig IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to guinea pig IgG may cross-react with immunoglobulins of other mammalian species if common binding sites are shared.
Suggested Working ConcentrationOptimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.
ELISA1:500 - 1:2500(1.0 μg/mL - 0.2 μg/mL)
Western Blot1:2500 - 1:10000 (0.2 μg/mL - 0.05 μg/mL)
Histo/Cytochemical1:100 - 1:250(5.0 μg/mL - 2.0 μg/mL)
ApplicationELISA,Blotting,Histo/Cytochemical Procedures
Affinity purified antibody isolated from a pool of
serum from goats immunized with purified guinea
pig IgG was labeled with peroxidase by the
periodate method of Nakane and Kawaoi.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。