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Gendepot/Xpert Total Histone Extraction Kit, H7200/H7200-100-100 ext

价格
¥6800.00
货号:H7200-100-100ext
浏览量:33
品牌:Gendepot
服务
全国联保
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商品描述
Uses: The Xpert Total Histone Extraction Kit is suitable for a quick preparation of total histone extracts from mammalian cells and tissue samples.
Input Amount: The minimal amount of starting materials can be as low as 10⁵ cells or 1mg of tissue. For the best results, the cell number should be greater than 10⁶ cells or the tissue amount should be greater than 10mg. A total of 100 standard extractions (use 10⁷ cells or per 100mg of tissue per extraction) can be performed with this kit.
Yield: Yield of the total histone proteins can be up to 0.4mg per 10⁷ cells or per 100mg tissue. The yield may vary depending on the cell or tissue type. Precautions: To avoid cross-contamination, carefully pipette the sample or sol ution into the strip wells. Use aerosol-barrier pipette tips and always change pipette tips between liquid transfers. Wear gloves throughout the entire procedure. In case of contact between gloves and sample, change gloves immediately.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。