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Gendepot/LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit, L0013/L0013-010-100 rxns

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¥3960.00
货号:L0013-010-100rxns
浏览量:95
品牌:Gendepot
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商品描述

TheLucyQDuo-Luciferase(Firefly&Renilla)AssayKitprovidesanefficientmeansofperformingtworeporterassays.IntheLucyQDuo-Luciferase(Firefly&Renilla)Assay,theactivitiesoffirefly(Photinuspyralis)andRenilla(Renillareniformisorseapansy)luciferasesaremeasuredsequentiallyfromasinglesample.

ThefireflyluciferasereporterismeasuredfirstbyaddingFLucworkingsolutiontogeneratealuminescentsignallastingatleastoneminute.Afterquantifyingthefireflyluminescence,thisreactionisquenched,andtheRenillaluciferasereactionisinitiatedsimultaneouslybyaddingRLucworkingsolutiontothesamesample.Bothassayscanbecompletedinabout4secondsusingaluminometerwithreagentauto-injectors.

Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。
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/**/
HighSsensitivityReagentsareoptimizedforgreatestsensitivityofFireflyandRenillaluciferasesignals
LowBackgroundVerylowbackgroundluminescence.NosubtractionisrequiredfromreADIngs.
ReproducibilityReliable,linearresultsforaconcentrationrangeoverseveralordersofmagnitude.
CompatibilityCompatIBLewithPromega\\\"sFlucandRlucvectors.
VersatilityDesignedforassayswithmanydifferenteukaryoticcelltypes(e.g.mammalian,lowerinvertebrates,etc.)usingmicro-platesorsingle-tubeluminometers