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Gendepot/amfiEco Taq DNA Polymerase,1 unit/ul, P0701/25x500 Units/P0701-102

价格
¥20400.00
货号:P0701-102
浏览量:80
品牌:Gendepot
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商品描述
ContentsamfiEco Taq DNA Polymerase, 1u/ulamfiEco 2X Reaction Buffer
ApplicationRoutine Robust PCR

amfiEcoTaq DNA Polymeraseis a thermostable DNApolymerase that possesses a 5' -> 3' polymerase activityand a double-strand specific 5' ->3' exonuclease activity.2X amfiEco PCR Master buffer including dNTPs andMg++ minimises reagents handling steps and reducethe risk of contamination.

To perform PCR, simply add water, primers and templateto resuspend the reaction mixture, and cycle.For addedconvenience,amfiEcoTaq DNA Polymerasecontainsred and yellow loading dyes to allow loading of PCRproduct directly on a gel after thermal cycling, minimizingpipetting steps and providing easy visualization of sample.The red dye runs in a range between 500bp (2% gel) and1500bp(0.8% gel) and the yellow dye runs at less than10bp.

Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。